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On the identical time, measurements of mechanical compression and nano-indentation (a generally applied means of testing the mechanical properties of supplies by indenting the check materials with a diamond tip while measuring the power-displacement response) may also present excessive-decision measurements of matrix and tissue elasticity, as a result of nano-indentation can obtain lateral resolutions of ∼15 μm ( Akhtar et al., 2009b ). However, a standard drawback with all of these techniques is that the tactic of interrogation can significantly have an effect on outcome, so cautious experimental design should be implemented; stiffness values are typically calculated as a perform of experimental measurements and rely precisely on how and which variables are measured.